Peptide Reconstitution Guide

Reconstitution Math

Reconstitution means dissolving milligrams (mg) of peptide into a solvent volume, then matching the resulting concentration to the markings on a standard U-100 insulin syringe (1 mL = 100 units).

Formulas

Example Calculation

To reconstitute a 5 mg vial using 2 mL of bacteriostatic water for a target dose of 0.25 mg:

1. Find Concentration:
   5 mg ÷ 2 mL = 2.5 mg/mL
2. Determine Volume:
   0.25 mg ÷ 2.5 mg/mL = 0.10 mL
3. Convert to Syringe Units:
   0.10 mL × 100 = 10 units

Video Walkthroughs

• Peptide Reconstitution Instructions (00:00:02)

  Demonstrates proper technique for handling lyophilized powder: sanitizing rubber stoppers with alcohol wipes, equalizing air pressure in the bacteriostatic water vial, slowly directing solvent down the inside glass wall (avoiding direct impact on the powder), and gently rolling the vial to dissolve without shaking.

• Peptide Calculator - How to Use a Peptide Calculator for Accurate Dosing (00:00:00)

  Shows how to map mathematical variables—syringe size (0.3 mL, 0.5 mL, or 1 mL), vial quantity (mg), solvent added (mL), and target dose (mg)—into an interactive tool that highlights the exact unit tick line on a U-100 syringe.

Top Reconstitution Calculators

• R&D Systems Reconstitution Calculator

  A standard laboratory calculator (formerly Tocris Bioscience) that calculates required solvent volumes based on compound mass and desired final concentrations (mg/mL or µg/mL).

• Bio-Techne Reconstitution Calculator

  Highly reliable tool for calculating mass-to-volume conversions and precise liquid ratios.